rabbit anti-human yap1 polyclonal antibody (Proteintech)
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Proteintech
rabbit anti-human yap1 polyclonal antibody
Rabbit Anti Human Yap1 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-human+yap1+polyclonal+antibody/anti+yap1/pmc07920586-74-50-56
Average 90 stars, based on 1 article reviews
Rabbit Anti Human Yap1 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-human+yap1+polyclonal+antibody/anti+yap1/pmc07920586-74-50-56
Average 90 stars, based on 1 article reviews
rabbit anti-human yap1 polyclonal antibody - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis"
Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis
Journal: OncoTargets and therapy
doi: 10.2147/OTT.S292287
Figure Legend Snippet: Primer Sequences
Techniques Used: Sequencing
Figure Legend Snippet: ANXA1 and Yap1 are involved in many of the same biological processes (BP) and play an important role in LN metastasis of HSCC. ( A ) BP GO terms of ANXA1; ( B ) KEGG pathway enrichment analysis. ( C ) Some of the BP GO of Yap1; ( D ) PPI network of genes involved in the cancer biological processes with both ANXA1 and Yap1.
Techniques Used:
Figure Legend Snippet: Down-regulation of ANXA1 decreases Yap1 protein levels. ( A and B ) The effects of ANXA1 silencing on Yap1 mRNA and protein levels were quantified by qRT-PCR and Western blotting; ( C ) mRNA expression levels of ANXA1 in HSCC tissues (10 samples from patients with LN metastasis,10 samples from patients without LN metastasis); ( D ) Yap1 protein expression was detected by Western blotting in HSCC patients (left) and quantitated relative to GAPDH (6 samples from patients with LN metastasis patients, 6 samples from patients without LN metastasis); ( E ) Pearson correlation analysis of ANXA1 and Yap1 expression levels. The expression of ANXA1 mRNA is used as the X-axis after logarithmic transformation. Similarly, Yap1 is represented on the Y-axis.
Techniques Used: Quantitative RT-PCR, Western Blot, Expressing, Transformation Assay
Figure Legend Snippet: Yap1 over-expression reverses the effects of ANXA1 silencing on FaDu cells. ( A ) The transfection efficiency of lentivirus was observed by fluorescence microscope. GFP and cherry represent the fluorescence intensity of sh-ANXA1 and oe-Yap1, respectively. Fluorescence micrograph (100 ×); ( B ) qRT-PCR was used to detect the mRNA expression levels of ANXA1 and Yap1. GAPDH was used as reference; ( C ) The expression of ANXA1 and Yap1 protein levels were detected by WB. GAPDH was used as internal control; ( D ) CCK8 staining assay was used to detect the cell proliferation activity for 4 days.; ( E ) Colony formation assay was used to detect the cell proliferation; ( F ) Transwell assay was used to determine the migration and invasion abilities of FaDu cells (200 × magnification; image acquired after 24 h); ( G and H ). Three days after transfection of Yap1, the apoptosis rate ( G ) and cell cycle status ( H ) of FaDu cells were assessed by FCM. Data are presented as mean ± SD of triplicates.
Techniques Used: Over Expression, Transfection, Fluorescence, Microscopy, Quantitative RT-PCR, Expressing, Control, Staining, Activity Assay, Colony Assay, Transwell Assay, Migration
Related Articles
Membrane:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Sequencing:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Quantitative RT-PCR:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Western Blot:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Expressing:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Transformation Assay:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Over Expression:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Transfection:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Fluorescence:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Microscopy:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Control:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Staining:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Activity Assay:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Colony Assay:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Transwell Assay:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); Migration:Article Title: Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcinoma with Lymph Node Metastasis Article Snippet: The same amount of total protein was separated on a 10% SDS-PAGE, transferred to a PVDF membrane and blocked with 5% skim milk at room temperature for 1 h. Membranes were probed with the following primary antibodies overnight at 4°C: rabbit anti-human ANXA1 monoclonal antibody (1:1000, CST, Boston, Massachusetts USA); |
